esf921 delta series methionine deficient medium  (Expression Systems Inc)

Journal: Communications Biology

Article Title: The spike of SARS-CoV-2 promotes metabolic rewiring in hepatocytes

doi: 10.1038/s42003-022-03789-9

Figure Lengend Snippet: a Western blot showing the modulation of ACE2 and NRP1 protein expression when incubated with methionine-choline deficient (MCD) medium for 6 and 24 h (along with their respective controls. Quantification of ACE2 or NRP1 protein expression by densitometry is also shown ( n = 3, unpaired t -test). b , c Western blot showing the expression of ACE2 and NRP1 protein expression when treated with 1 mM metformin for 6 or 24 h ( n = 3, unpaired t -test) ( b ) or with MCD or MCD plus 1 mM metformin for 10 h ( n = 3, one-way ANOVA) ( c ) as well as their respective controls. Relative quantification of ACE2 or NRP1 protein expression (related to β-actin) is also shown. d Relative infection with pseudotyped viral particles or controls of WT or hACE2 mouse hepatocytes (left) or upcyte second-generation human hepatocytes (right) upon treatment with MCD or MCD and metformin, measured by flow cytometry (one-way ANOVA). e Extracellular levels of ANG(1–7) secreted by human primary hepatocytes after infection with pseudotyped viral particles upon treatment with MCD or MCD and metformin, measured by ELISA ( n = 5, one-way ANOVA). Error bars represent SEM and asterisks represent p values (*<0.05, **<0.01, and ***<0.001).

Article Snippet: Steatotic conditions were induced in primary hepatocytes as follows: incubation overnight on 0%-FBS MEM medium containing PSG followed by incubation in methionine-choline deficient (MCD) medium (Gibco Cat#: ME120128L1) supplemented with PSG.

Techniques: Western Blot, Expressing, Incubation, Infection, Flow Cytometry, Enzyme-linked Immunosorbent Assay